TIGAR, Human, Control Peptide (Tp53-Induced Glycolysis and Apoptosis Regulator)

The p53 tumor-suppressor gene integrates numerous signals that control cell life and death; loss of its functions contributes to the development of most cancers (1). Recent studies have demonstrated the ability of p53 to regulate the expression of several proteins involved in glycolysis and oxidative phosphorylation, such as TIGAR, SCO2, and phosphoglycerate mutase (2). TIGAR is a recently discovered protein that functions to regulate glycolysis and protect cells against oxidative stress (3). TIGAR is similar in structure to proteins in the phosphoglycerate mutase family, most notably 6-phosphofructo-2-kinase, suggesting TIGAR may function as a fructose bisphosphatase. Expression of TIGAR in transfected cells correlated with an inhibition of glycolysis and decreased levels of reactive oxygen species and p53-induced apoptosis, indicating that TIGAR may act to modulate the apoptotic response to p53, thereby allowing cells to survive mild or transient stresses. \n\nLocation:\n19 amino acids near the center of human TIGAR\n\nApplications:\nPeptide is used for blocking the activity of TIGAR antibody (T5499-02). It usually blocks the antibody activity completely in Western Blot by incubating the peptide with equal volume of antibody for 30 min at 37?C. Buffer is PBS containing 0.02% sodium azide. Other applications not tested.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5499-02A

Size

50ug

Applications

WB

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% BSA and 0.02% sodium azide.

Purity

Purified

References

1. Guimaraes DP and Hainaut P. TP53: a key gene in human cancer. Biochimie 2002; 84:83-93.\n2. Corcoran CA, Huang Y, and Sheikh MS. The regulation of energy generating pathways by p53. Cancer Biol. Ther. 2006; 5:1610-3.\n3. Bensaad K, Tsuruta A, Selak MA, et al. TIGAR, a p53-inducible regulator of glycolysis and apoptosis. Cell 2006; 126:107-20.