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TIGAR (Tp53-Induced Glycolysis and Apoptosis Regulator, Chromosome 12 Open Reading Frame 5, C12orf5, Probable Fructose-2,6-bisphosphatase TIGAR)

Cat no: T5499-01B

TIGAR (Tp53-Induced Glycolysis and Apoptosis Regulator, Chromosome 12 Open Reading Frame 5, C12orf5, Probable Fructose-2,6-bisphosphatase TIGAR)

TIGAR is a p53-inducible protein that functions to regulate glycolysis and protect against oxidative stress and is part of the p53 tumor suppressor pathway. It encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2, 6-bisphosphate. TIGAR functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Overexpression of TIGAR in mouse and human cells resulted in inhibition of glycolysis, as well as a decrease in intracellular reactive oxygen species (ROS) and provides some protection from DNA damage-induced apoptosis in a cell type-specific manner.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMCF7\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T5499-01B

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Can, NHP

Form

Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Bensaad, K. et al. Cell. 126:107-120 (2006). 2. Jen, KY. et al. Cancer Res. 65:7666-7673 (2005).

Additional Info

Recognizes human TIGAR (TP53-induced glycolysis and apoptosis regulator). Species Crossreactivity: chimpanzee, canine, monkey.

Alternative Names

EC=3.1.3.46

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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