TIM-1 (T Cell Immunoglobin Domain and Mucin Domain Protein 1, TIM 1, TIM1, TIMD 1, TIMD1, TIMD-1, Hepatitis A Virus Cellular Receptor 1, HAVCR 1, HAVCR1, HAVCR-1, Kidney Injury Molecule 1, KIM1, KIM-1)

TIM-1, also known as HAVcr1 (C Hepatitis A virus cellular receptor 1) and KIM-1 (Kidney Injury Molecule 1), is a 60-100kD member of the TIM family of immune regulating adhesion proteins. The mature molecule is a type I transmembrane glycoprotein that contains a 214aa extracellular domain (ECD) and a 51aa cytoplasmic tail. The ECD contains one Ig-like V-type domain, a Ser/Thr-rich mucin region, and multiple glycosylation sites. TIM-1 is involved in the development of Th2-biased immune responses, and the presence of particular TIM-1 mutations correlates with the susceptibility to allergic disease. TIM-1 binds TIM-4 and serves as the receptor for the hepatitis A virus. It is also upregulated in renal epithelial cells following tissue injury. The extracellular domain of TIM-1 can be shed and serves as an indicator of kidney tubule damage. Within the ECD, rat TIM-1 shares 81% and 51% aa sequence identity with mouse and human TIM-1, respectively.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman or mouse brain tissue lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T5469-91F

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity Chromatography.

Isotype

IgG

References

1. Feigelstock,D., Thompson,P., Mattoo,P., Zhang,Y. and Kaplan,G.G. The human homolog of HAVcr-1 codes for a hepatitis A virus cellular receptor. J. Virol. 72 (8), 6621-6628 (1998). 2. Vila,M.R., Kaplan,G.G., Feigelstock,D., Nadal,M., Morote,J., Porta,R., Bellmunt,J. and Meseguer,A. Hepatitis A virus receptor blocks cell differentiation and is overexpressed in clear cell renal cell carcinoma. Kidney Int. 65 (5), 1761-1773 (2004). 3. McIntire,J.J., Umetsu,D.T. and DeKruyff,R.H. TIM-1, a novel allergy and asthma susceptibility gene. Springer Semin. Immunopathol. 25 (3-4), 335-348 (2004). 4. Chae,S.C., Song,J.H., Heo,J.C., Lee,Y.C., Kim,J.W. and Chung,H.T. Molecular variations in the promoter and coding regions of human Tim-1 gene and their association in Koreans with asthma. Hum. Immunol. 64 (12), 1177-1182 (2003). 5. McIntire, JJ et al. Nature Immunol. 2, 1109-1116 (2001) 6. McIntire, JJ et.al. Nature 425(6958):576 (2003 Oct 9) 7. V.Bailly et.al J. Biol. Chem., Vol. 277, Issue 42, 39739-39748 (October 18, 2002) 8. Umetsu D, S Umetsu, GJ Freeman, RH Dekruyff. TIM gene family and their role in atopic disease. Curr Top Microbiol Immunol 208:201-215. (2008).

Additional Info

Recognizes human HAVCR1/TIM-1. Species Crossreactivity: mouse. Species sequence homology: monkey.