Timeless (Tim1) \n

Several endogenous factors have been linked to rhythmicity or circadian behavior of living organisms. The genes period (dPer) and timeless (tim) in Drosophila and frequency (frq) in Neurospora have been proposed to be responsible for their circadian rhythm. In mammals, the suprachiasmatic nuclei (SCN) of the anterior hypothalamus serves as a master circadian clock with a measurable circadian rhythm. In Drosophila, the RNA and protein products of per and tim oscillate and may be involved in rhythm regulation. The levels of per and tim is positively regulated by two basic helix-loop-helix (bHLH)/PAS proteins, dCLOCK and dBMAL1, which heterodimerize and bind to E box enhancers. Translated per and tim are phosphorylated, form heterodimers, and then translocate to the nucleus. Once in the nucleus, PER and TIM function as negative regulators of their own transcription. Recently drosophila homologs of mammalian TIM have been cloned and characterized (1). Human Timeless 1 (hTIM1; 1208aa, chromosome 12q12-13) and mouse TIM1 (1197aa, chromosome 10D3) share 83% identity. Tim1 is weakly expressed in SCN, and in several peripheral tissues (eyes, spleen and testis). Unlike Drosophila, mouse Tim1 does not oscillate in the SCN and is not modulated by light exposure. Human TIM1 and mPER1 specifically inhibit CLOCK-BMAL1-induced transactivation of mPER1 promoter.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. See T5470-02.\nWestern Blot: 1-10ug/ml using ECL.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5470-03

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Sangoram, A.M., et al., Neuron 21: 1101-1113 (1998). 2. Zylka, M.J., et al., Neuron 21: 1115-1122 (1998). 3. Koike, N., et al., FEBS Lett. 441: 427-431 (1998).

Additional Info

Recognizes mouse and human (Tim1). Species Sequence Homology (Mouse Tim1 peptide): Rat (100%), Drosophila (85%), Human (85%). Species Sequence Homology (Human Tim1 peptide): Mouse (57%). Expected to crossreact with rat and Drosophila based on sequence homology.