TIMM50 (Translocase of Inner Mitochondrial Membrane 50 Homolog (Yeast), TIM50, Homolog of Yeast Tim50, Import Inner Membrane Translocase Subunit TIM50, Import Inner Membrane Translocase Subunit TIM-50 Mitochondrial, MGC102733, Tim50-like Protein, TIM50L)

TIMM50 is a component of the yeast Tim23 import machinery, which mediates translocation of presequence containing proteins across the mitochondrial inner membrane. By searching sequence databases, open reading frames encoding proteins with similarity to Tim50 and with a putative mitochondrial presequence were identified in the genomes of evolutionarily distant organisms, including human. Tim50 was found to interact with the N terminal intermembrane space domain of Tim23. Functional defects of Tim50 either by depletion of the protein or addition of anti Tim50 antibodies blocked the protein translocation across the inner membrane. A translocation intermediate accumulated at the translocator of the outer mitochondrial membrane (TOM) complex was cross linked to Tim50. Thus it can be concluded that Tim50, in cooperation with Tim23, facilitates transfer of the translocating protein from the TOM complex to the Tim23 complex.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman Heart lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T5472-50A

Size

100ug

Applications

WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Guo Y, Cheong N, Zhang Z, De Rose R, Deng Y, Farber SA, Fernandes-Alnemri T, Alnemri ES.Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death.J Biol Chem. 2004 Jun 4;279(23):24813-25. Epub 2004 Mar 25. PMID: 15044455.

Additional Info

Recognizes human TIM50. Species sequence homology: mouse.