TIMP-1 BioAssay(TM) ELISA Kit, Rat

Sandwich ELISA quantitative detection kit for Rat TIMP-1 in cell culture supernates, serum and plasma (heparin, EDTA).\n\nThe tissue inhibitor of metalloproteinases 1 (TIMP1) is also called erythroid-potentiating activity (EPA). The X-linked gene for human TIMP1 is expressed in some but not all inactive X-containing somatic-cell hybrids, suggesting that this gene is either prone to reactivation or variable in its inactivation.1 Purified EPA specifically stimulates human and murine cells of the erythroid lineage, unlike murine interleukin-3 (IL-3) which stimulates precursor cells from all haematopoietic lineages.2 TIMP1 is thought to play a regulatory role in connective tissues by forming inactive complexes with those metalloproteinases that are normally responsible for connective tissue turnover. The human gene encoding TIMP has been mapped to the X chromosome in the region Xp11.1-p11.4.3 The standard product used in this kit is recombinant rat TIMP-1, consisting of 194 amino acids with the molecular mass of 21.5kD. As a result of glycosylation, the molecular mass is 32-34kD.\n\nAssay Range:\n31.2pg/ml-2000pg/ml\n\nSensitivity:\n< 3 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP30120\n\nEntrez Gene ID:\n116510\n\nKit Components:\n96-well plate pre-coated with anti-TIMP-1, Rat\nLyophilized recombinant TIMP-1, Rat standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

144057

Size

96Tests

References

1. Anderson, C. L.; Brown, C. J. Polymorphic X-chromosome inactivation of the human TIMP1 gene. Am. J. Hum. Genet. 65: 699-708, 1999. \n2. Gasson, J. C.; Golde, D. W.; Kaufman, S. E.; Westbrook, C. A.; Hewick, R. M.; Kaufman, R. J.; Wong, G. G.; Temple, P. A.; Leary, A. C.; Brown, E. L.; Orr, E. C.; Clark, S. C. Molecular characterization and expression of the gene encoding human erythroid-potentiating activity. Nature 315: 768-771, 1985. \n3. Spurr, N. K.; Goodfellow, P. N.; Docherty, A. J. P. Chromosomal assignment of the gene encoding the human tissue inhibitor of metalloproteinases to Xp11.1-p11.4. Ann. Hum. Genet. 51: 189-194, 1987. \n