TIMP-2 BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human TIMP-2 in cell culture supernates, serum, plasma (heparin, EDTA) and saliva.\n\nTIMP2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22kD and 194 amino acid.\n\nAssay Range:\n156pg/ml-10,000pg/ml\n\nSensitivity:\n< 2 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP16035\n\nEntrez Gene ID:\n7077\n\nKit Components:\n96-well plate pre-coated with anti-TIMP-2, Human\nLyophilized recombinant TIMP-2, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

144058

Size

96Tests

References

1. De Clerck, Y.; Szpirer, C.; Aly, M. S.; Cassiman, J.-J.; Eeckhout, Y.; Rousseau, G. : The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. Genomics 14: 782-784, 1992.\n2. Hammani, K.; Blakis, A.; Morsette, D.; Bowcock, A. M.; Schmutte, C.; Henriet, P.; DeClerck, Y. A. : Structure and characterization of the human tissue inhibitor of metalloproteinases-2 gene. J. Biol. Chem. 271: 25498-25505, 1996.\n3. Seo, D.-W.; Li, H.; Guedez, L.; Wingfield, P. T.; Diaz, T.; Salloum, R.; Wei, B.; Stetler-Stevenson, W. G. : TIMP-2 mediated inhibition of angiogenesis: an MMP-independent mechanism. Cell 114: 171-180, 2003.\n