TIMP-2, Mouse, BioAssay(TM) ELISA Kit

Mouse TIMP-2 ELISA (Enzyme-Linked Immunosorbent Assay) BioAssay(TM) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse TIMP-2. This assay employs an antibody specific for mouse TIMP-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse TIMP-2 antibody is added. After washing away unbound biotinylated antibody, HRP- conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm. The minimum detectable dose of TIMP-2 is typically less than 80pg/ml. \n\nSource:\nMouse\n\nSample Type:\nSerum, plasma and cell culture supernatants.\n\nIntended Use:\nThe BioAssay(TM) kit is used for quantitative protein detection, establishing normal range and validation of antibody array results. The antibody pair provided in this kit recognizes mouse TIMP-2.\n\nSensitivity:\nThe intra-Assay reproducibility is CV<10% and inter-Assay is CV<12%. \n\nKit Components:\nTIMP-2 Microplate (Item A), 1x96wells\nWash Buffer Concentrate, 1x25ml\nStandards (Item C), 2x vials\nAssay Diluent A (Item D), 1x30ml\nAssay Diluent B (Item E), 1x15ml\nDetection Antibody TIMP-2 (Item F), 2x vials\nHRP-Streptavidin Concentrate (Item G), 1x200ul\nTMB One-Step Substrate Reagent (Item H), 1x12ml\nStop Solution (Item I), 1x8ml\n\nStorage and Stability:\nStore powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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