Tin2 (TERF1-interacting Nuclear Factor 2, TERF1 (TRF1)-interacting Nuclear Factor 2, TRF1-interacting Nuclear Factor 2, TINF2)

TRF1 is a negative regulator of telomere length maintenance (3). Recently two proteins, tankyrase and Tin2, which bind to mammalian TRF1, have been identified. Tankyrase is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate-ribose) polymerase (PARP) (3). Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats (2,3). Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP)-ribosylation. Since ADP-ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP)-ribosylation. Both the cell cycle and TRF1 may regulate the subcellular localization of tankyrase. The cDNA coding for TIN2 protein was isolated as interacting partner of TRF1 from a yeast two-hybrid cDNA library screening (1). Tin2 localizes to telomeres and is essential for proper regulation of telomere length. TIN2, like TRF1, is widely and constitutively expressed, suggesting that these proteins act together to counterbalance telomere elongation by telomerase. However, endogenous TIN2, like endogenousTRF1, is not highly expressed, and therefore may be difficult to visualize by immunostaining in certain cell systems.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nCellular Localization: Nuclear\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5475-01A

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Hum

Form

PBS, 0.2% gelatin, 0.05 % sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

Smith S & de Lange T Cell cycle dependent localization of the telomeric PARP, tankyrase, to nuclear pore complexes and centrosomes. J Cell Sci 112 ( Pt 21):3649-56 (1999). <PUBMED:10523501>Kim SH et al. TIN2, a new regulator of telomere length in human cells. Nat Genet 23:405-12 (1999). <PUBMED:10581025>Smith S et al. Tankyrase, a poly(ADP-ribose) polymerase at human telomeres. Science 282:1484-7 (1998). <PUBMED:9822378>

Additional Info

Species Crossreactivity: Crossreacts with Human. Expected to crossreact with Mouse (87% identity with immunogen) due to sequence homology. Not yet tested in other species.