While both TIP120A and TIP120B are TATA binding proteins and form complexes with various nuclear proteins involved in the control of eukaryotic gene transcription, TIP120B is expressed specifically in muscle and heart tissue. This is contrary to the ubiquitous expression of TIP120A. TIP120 homologs exist in various higher eukaryotes including D. melanogaster, C. elegans, and A. thaliana. TIP120B is 60% identical in aa sequence to TIP120A.
Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 1:2,000-1:10,000
Western Blot: 1:500-1:1,000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.