Tip60

Acetylation is a prominent post-translational modification of nucleosomal histone N-terminal tails, which regulates chromatin accessibility. Accordingly, histone acetyltransferases (HATs) play major roles in processes such as transcription. It has been shown that the HAT Tip60, which is involved in DNA repair and apoptosis following gamma irradiation, is subjected to proteasome-dependent proteolysis. Furthermore, we provide evidence that Mdm2, the ubiquitin ligase of the p53 tumor suppressor, interacts physically with Tip60 and induces its ubiquitylation and proteasome-dependent degradation. Moreover, a ubiquitin ligase-defective mutant of Mdm2 had no effect on Tip60 stability. Results indicate that Mdm2 targets both p53 and Tip60, suggesting that these two proteins could be co-regulated with respect to protein stability. Consistent with this hypothesis, Tip60 levels increased significantly upon UV irradiation of Jurkat cells. Collectively, results suggest that degradation of Tip60 could be part of the mechanism leading to cell transformation by Mdm2.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested. \n\nRecommended Dilution:\nImmunoblot Analysis: 0.5-2ug/ml of this lot detected Tip60 in RIPA lysates from Cos-1 cells transfected with a human Tip60 cDNA.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5499-94

Size

200ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before to the addition of glycerol to 30%.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Brady, M.E., et al., J. Biol. Chem. 274: 17599-17604, 1999.EMBO J. 2002 Apr2;21(7):1704-12.Tip60 is targeted to proteasome-mediated degradation by Mdm2 and accumulates after UV irradiation. Legube G, Linares LK, Lemercier C, Scheffner M, Khochbin S, Trouche D.

Additional Info

Recognizes Tip60, Mr 60kD. Additional proteins were detected at 66kD and 80kD in some human and mouse cell lines. Species Crossreactivity: Human and mouse.