TIRAP, CT (Toll-interleukin 1 Receptor Domain Containing Adapter Protein, TIR Domain-containing Adapter Protein, Adaptor Protein Wyatt, MyD88 Adapter-like Protein, Mal, Tlr4ap)

Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as TIRAP and MyD88 to activate various kinases and transcription factors. In TIRAP-deficient mice, TLR signaling in response to TLR2 ligands (using either TLR1 and TLR6 as co-receptors) is totally abolished, suggesting that MyD88 and TIRAP work together and are both required for TLR2 signaling. Furthermore, these mice are also resistant to the toxic effects of LPS and show defects in NF-?B and MAP kinase activation, suggesting that TIRAP is also involed in TLR4 signaling.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml\nImmunohistochemistry (paraffin): 2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman heart cell lysates.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T5500-01M

Size

50ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Vogel SN, Fitzgerald KA, and Fenton MJ. TLRs: differential adapter utilization by toll-like receptors mediates TLR-specific patterns of gene expression. Mol. Interv. 2003; 3:466-77. 2. Takeda K, Kaisho T, and Akira S. Toll-like receptors. Annu. Rev. Immunol. 2003; 21:335-76. 3. Janeway CA Jr and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216. 4. O

Additional Info

Recognizes mouse TIRAP/Mal (CT). Species Crossreactivity: rat and human.

Alternative Names

Toll-interleukin 1 Receptor (TIR) Domain-containing Adaptor Protein, AA407980, C130027E04Rik