TIRAP (MAL, Toll-interleukin 1 Receptor Domain containing Adaptor Protein, TIRAP)

Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses (1-3). These proteins act through adaptor molecules such as TIRAP and MyD88 to activate various kinases and transcription factors (4,5). In TIRAP-deficient mice, TLR signaling in response to TLR2 ligands (using either TLR1 and TLR6 as coreceptors) is totally abolished, suggesting that MyD88 and TIRAP work together and are both required for TLR2 signaling (6). Furthermore, these mice are also resistant to the toxic effects of LPS and show defects in NF-kB and MAP kinase activation, suggesting that TIRAP is also involed in TLR4 signaling (6,7).\n\nApplications:\nCan be used for detection of TIRAP by Western blot at 4ug/ml.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T5500-01

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Ion Exchange chromatography.

Isotype

IgG

References

1. Vogel SN,et al . Mol. Interv. 2003; 3:466-77. 2. Takeda K, et al Annu. Rev. Immunol. 2003; 21:335-76. 3. Janeway CA Jr and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216. 4. O'Neill LAJ, et al Trends in Imm. 2003; 24:286-9. 5. McGettrick AF and O'Neill LAJ. Mol Imm. 2004; 41:577-82. 6. Yamamoto Y, et al Nature 2002; 420:324-9. 7. Hoorng T, Barton GM, FlavellRA, et al. The adaptor molecule TIRA P provides signalling specificity for Toll-like receptors. Nature 2002; 420:329-333.

Additional Info

Recognizes human TIRAP.