TIS7 (TPA-induced Sequence 7, TIS7 Protein, Interferon-related Developmental Regulator 1, IFRD1, Nerve Growth Factor-inducible Protein PC4, PC4)

Tis7, also known as IFRD1, is a 442aa containing membrane associated, non-nuclear intracellular protein belonging to the IFRD family. A regulatory protein expressed in a variety of tissues, Tis7 is known to play an important role in the regulation of gene activity in the proliferative and/or differentiative pathways induced by NGF. Reports suggest that Tis7 may be an autocrine factor that attenuates or amplifies the initial ligand induced signal and might also augment the adaptive response by stimulating the production of differentiated enterocytes. Tis7 acts as a negative regulator of transcriptional activity and represses expression of genes involved in myogenesis, muscle maintenance, and regeneration in a histone deacetylase dependent manner. \n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-4ug/ml\nImmunohistochemistry (formalin fixed paraffin embedded): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T5565-50

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Bov, Can, Moll, Prc

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

1. Varnum, BC. et al. Oncogene. 4:1263-1265 (1989). 2. Vietor, I. et al. J Biol Chem. 280:39795-39801 (2005). 3. Vietor, I. et al. Differentiation. 75:891-897 (2007).

Additional Info

Recognizes human Tis7. Species Crossreactivity: chimpanzee, bovine, canine, macaque, pocine, rhesus monkey. Species sequence homology: mouse, rat, equine, xenopus, chicken, opossum.