BioSelect(TM) Tissue cDNA: cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection.
PCR Ready First Strand cDNAs is an excellent source of tissue specific, PCR-ready cDNA, and it can be immediately used for gene discovery or expression analysis. First-Strand cDNA is synthesized from RNA isolated from documented human adult normal tissues. Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 11ug total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40ul final volume.
RT Reaction stopped by heating at 65 degrees C for 10 minutes.
Features:
Ready to use for PCR
Oligo dT primer used to ensure the entire 3' end of cDNA is present
With some cDNA used as templates, 12kb PCR amplicon was obtained to ensure the intactness of cDNA
Applications:
Immediate PCR Amplification of known genes. Verification of genetic mutation
Comparison of a specific gene between different tissues. Analysis of mRNA alternative splicing Gene cloning and target sequencing
Contents:
10ul per package
Form:
Supplied as a liquid in 1X RT buffer. (1X RT Buffer: 50 mM Tris-Cl, pH 8.3, 75mM KCl, 3mM MgCI2, 10mM DTT).
Concentration:
~2.5-5.0ng/ul
Unit:
1ul cDNA is good for one PCR reaction.
Storage Conditions:
Store cDNAs at -20 degrees C
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