Tissue cDNA, First Strand, Human Diseased (Adult), Parkinson

BioSelect(TM) Tissue cDNA: cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection.\n\nPCR Ready First Strand cDNAs is an excellent source of tissue specific, PCR-ready cDNA, and it can be immediately used for gene discovery or expression analysis. First-Strand cDNA is synthesized from RNA isolated from a wide variety of documented human adult and fetal normal tissues, human diseased and tumor tissues, mouse, rat, monkey, plant and other tissues. Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 11ug total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40ul final volume. RT Reaction stopped by heating at 65 degrees C for 10 minutes. \n\n1ul cDNA is good for one PCR reaction. The 5' end of human clathrin cDNA (a 6kb gene) has been amplified by PCR from all of the cDNAs. \n\nFeatures:\nReady to use for PCR \nOligo dT primer used to ensure the entire 3' end of cDNA is present \nWith some cDNA used as templates, 12kb PCR amplicon was obtained to ensure the intactness of cDNA \nThe largest selection of cDNAs from different tissues on the market \nDocumentation of tissues' clinical histories available (additional cost)\n\nApplications:\nImmediate PCR Amplification of known genes \nVerification of genetic mutation \nComparison of a specific gene between different tissues \nAnalysis of mRNA alternative splicing \nGene cloning and target sequencing \n\nQuality Control:\n1. The integrity of the RNA used for cDNA synthesis is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoresed on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10mM Tris-Cl, pH 7.5). The ratio of 28S/18S is (same/more than) 1.\n2. The RNA used for cDNA synthesis is treated by DNase I, and is tested as DNA free RNA by PCR.\n3. The synthesized human, animal, and cell line cDNA was 5

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