Tissue cDNA, First Strand, Human Tumor, Melanoma, BioGenomics(TM)

BioSelect(TM) Tissue cDNA: cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection.\n\nPCR Ready First Strand cDNAs is an excellent source of tissue specific, PCR-ready cDNA, and it can be immediately used for gene discovery or expression analysis. First-Strand cDNA is synthesized from RNA isolated from a wide variety of documented human adult and fetal normal tissues, human diseased and tumor tissues, mouse, rat, monkey and plant tissues. Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 11ug total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40ul final volume. RT Reaction stopped by heating at 65 degrees C for 10 minutes. The cDNA is in 1X RT buffer. (1X RT Buffer: 50 mM Tris-Cl, pH 8.3, 75mM KCl, 3mM MgCI2, 10mM DTT). \n\n1ul cDNA is good for one PCR reaction. The 5' end of human clathrin cDNA (a 6kb gene) has been amplified by PCR from all of the cDNAs. \n\nFeatures:\nReady to use for PCR \nOligo dT primer used to ensure the entire 3' end of cDNA is present \nWith some cDNA used as templates, 12kb PCR amplicon was obtained to ensure the intactness of cDNA \nThe largest selection of cDNAs from different tissues on the market \nDocumentation of tissues' clinical histories available (additional cost)\n\nApplications:\nImmediate PCR Amplification of known genes \nVerification of genetic mutation \nComparison of a specific gene between different tissues \nAnalysis of mRNA alternative splicing \nGene cloning and target sequencing \n\nContents: \n40ul or 10ul per package\n\nStorage Conditions:

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