BioSelect(TM) Tissue cDNA: cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection.
Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 11ug total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40ul final volume. RT Reaction stopped by heating at 65C for 10 minutes. The cDNA is in 1x RT buffer. (1x RT Buffer: 50mM Tris-CI, pH 8.3, 75mM KCI, 3mM MgCI2, 10mM DTT). The estimated cDNA concentration is about 2.5ng/ul.
1ul cDNA is sufficient for one PCR reaction.
Applications:
Immediate PCR Amplification of known genes. Verification of genetic mutation.
Comparison of a specific gene between different tissues. Analysis of mRNA alternative splicing Gene cloning and target sequencing
Storage Conditions:
Store cDNAs at -20 degrees C
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