BioSelect(TM) Tissue cDNA: cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection.
PCR Ready First Strand cDNAs is an excellent source of tissue specific, PCR-ready cDNA. It can be immediately used for gene discovery or expression analysis. First-Strand cDNA is synthesized from RNA isolated from documented human adult normal tissues. Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 11ug total RNA was primed by an oligo dT primer and reverse transcribed by MMLV reverse transcriptase in 40ul final volume.
RT Reaction stopped by heating at 65 degrees C for 10 minutes.
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