Tissue cDNA Library, Human Adult Normal, Spleen, BioGenomics(TM)

BioSelect(TM) Tissue cDNA: cDNA is supplied as First Strand, Multiple Tissue Panels, and Matched Pairs. PCR-ready First Strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. Over 350 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues are included in this extensive collection. Description: Our high quality cDNA libraries are constructed by using an oligo dT primer-adapter and Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) to prime and synthesize first strand cDNA from mRNA. After the second strand is synthesized, the double stranded cDNA is size fractionated, cloned directionally into our vector and transformed into T1 phage resistant E. coli. Average insert size and insert size range are determined by restriction enzyme digestion of 24 clones picked at random from each library. The 4 kb vector used for cloning is Puc based, confers ampicillin resistance and contains the CMV promoter for expression analysis. This vector also contains the SP6 and T7 RNA polymerase promoters flanking the MCS for RNA synthesis, the Amersham ET and M13 primer sites for sequencing and the F1 ori for single-stranded DNA production. By cloning the cDNA directionally into this vector the cDNA clones can be expressed, detected by antibody screening and the libraries can be used to produce normalized or subtracted libraries. Applications: cDNA probe for profiling study in gene expression, PCR amplification and Conventional library screening. Other applications not tested. Form: Supplied as a liquid in 80% SOC, 20% glycerol. Titer: 7.0 x 10e9 cfu/ml Quality Control: 1. Minimum number of primary clones: 10e6 2. Minimum percentage of recombinant clones: 87% 3. Minimum insert size: 1Kb 4. Average number of primary clones: 3x10e6 5. Average percentage of recombinant clones: 95% 6. Average insert size: 1.5Kb Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.