Tissue, DNA, RNA, Protein, Extraction Kit, BioAssay(TM)

Isolation from the same piece of tissue simultaneously\n\nDescription\nIn order to study the function of DNA, protein, RNA and their relationship, it is very important to get RNA, DNA and protein from the same piece of tissue. DNA, Protein, RNA Extraction Kit provides a convenient and efficiency system for isolation of RNA, DNA and protein from the same piece of tissue simultaneously. The isolated RNA can be used for mRNA isolation, probe generation, RT-PCR, Northern blot analysis, primer extension, RNA protection assay, and In vitro translation. The isolated DNA can be used for PCR amplification. And the isolated protein can be used for Western Analysis. The kit is enough for isolating DNA/RNA/Protein from 5 gram tissues or 10 gram cells or blood.\n\nQuality Control:\nA representative kit from the same lot is randomly selected for isolation of RNA, DNA and protein. The quality and purity of isolated total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10mM Tris-Cl, pH 7.5). The integrity of the RNA is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose gel. Beta-actin gene, 838 bp fragment, is successfully amplified from the isolated genomic DNA. And the isolated protein

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