Tissue Factor Pathway Inhibitor 2 (TFPI2)

TFPI-2 (tissue factor pathway inhibitor-2; also placental protein 5/PP5) is a secreted, presumably 27-34kD glycoprotein member of the Kunitz domain-containing proteinase inhibitor family. In mouse, it is principally produced by endothelium, hepatocytes and synctiotrophoblasts, and serves as an inhibitor of multiple serine proteinases. Mature mouse TFPI-2 is 208aa in length. It contains three Kunitz inhibitor domains, the most N-terminal of which (aa36-86) is crucial to inhibitor activity. Over aa23-211, mouse TFPI-2 shares 86% and 58% aa sequence identity with rat and human TFPI-2, respectively.\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation and ELISA. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1-0.2ug/ml. Detection limit for rmTFPI-2 is ~20 ng/lane and 2 ng/lane under non-reducing and reducing conditions.\nELISA (direct): 0.5-1ug/ml. Detection limit for rmTFPI-2 is ~0.1ng/well. \nImmunoprecipitation: Immunoprecipitates rmTFPI-2 from conditioned media of transfected NS0 cells. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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