Tissue Inhibitor of Metalloproteinases 2 (TIMP2) (BSA and Azide Free)

TIMP-2 is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kD Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92kD Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.\n\nApplications: \nSuitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: 5ug/ml (PLP fixed tissue and paraffin tissues), 1ug/ml (Frozen tissues)\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T5585-27X

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, GP, Rabbit

Form

Supplied as a liquid in PBS, pH 7.0.

P Type

Mab

Purity

Purified

Isotype

IgG1,k

References

1. Fujimoto, N. et al. (1993). A one-step sandwich enzyme immunoassay for tissue inhibitor of metalloproteinases-2 using monoclonal antibodies. Clin. Chim. Acta. 220: 31- 45. 2. Fujimoto, N. et al. (1995). Determination of tissue inhibitor of metalloproteinases-2 (TIMP-2) in experimental animals using monoclonal antibodies against TIMP-2 specific oligopeptides. J. Immunol. Methods. 187: 33

Additional Info

Recognizes human TIMP-2. Species Crossreactivity: mouse, rat, guinea pig, rabbit and bovine.