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Tissue, MicroRNA Isolation Kit, BioAssay(TM)

Cat no: T5595-2710M

Tissue, MicroRNA Isolation Kit, BioAssay(TM)

Description:\nMicroRNA Isolation Kit provides a rapid method for the isolation and purification of small RNA molecules (< 200nt) from tissue culture cells and small tissues. This kit can also be used for isolating large RNA (> 200nt). This kit contains enough reagents for 25 isolations of small RNAs (< 200nt) or 50 isolations of large RNAs (> 200nt).\n\nQuality Control:\nA representative kit from the same lot is randomly selected for isolation of small RNAs from cultured cells and tissues. The quality and purity of isolated small RNAs were measured by denaturing agarose gel electrophoresis and spectrophotometer. A260/280 is between 1.8 and 2.0 (measured in 10mM Tris-Cl, pH 7.5). The presence of miRNAs in the isolated small RNAs was detected using MicroRNA qRT-PCR Detection Kit.\n\nColumn binding capacity 100ug\nRecommended amount of starting material for one isolation: \nTissues 0.5 пїЅ 200mg \nTissue culture cells 100 пїЅ 1x 10e7\nBlood and serum 200ul\nMaximum column loading volume 650ul\nElution Volume 30пїЅ100ul

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SPECIFICATIONS

Catalog Number

T5595-2710M

Size

1Kit

References

1. Liu, C.G., et al., Proc. Natl. Acad. Sci. USA 101(26): 9740-9744 (2004). 2. Lim, L.P., et al., Science 299(5612): 1540 (2003). 3. Zeng, Y., et al., Proc. Natl. Acad. Sci. USA 100(17): 9779-9784 (2003). 4. Boom, R., et al., Journal of Clinical Microbiology 23(3): 495-503 (1990). 5. Chomczynski, P. & Sacchi, N., Analytical Biochemistry 162: 156-159 (1987).

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