mRNA is isolated from total RNA obtained from human adult and fetal normal tissues, human tumor and diseased tissues, as well as from mouse, rat, monkey, and plant tissues. The RNA undergoes two rounds of oligo(dT) selection utilizing proprietary technology to minimize the presence of ribosomal RNA. Potential DNA contaminants are removed by a stringent DNase treatment. Contamination by RNase, DNA polysaccharides and proteoglycans has been effectively eliminated. The mRNA is purified by affinity chromatography using oligo dT columns. The purity and intactness of the mRNA is verified by agarose gel electrophoresis, Northern analysis, cDNA synthesis, and capillary electrophoresis using a human beta-actin cDNA probe.
Features:
mRNA isolated from a wide variety of tissues and cells. DNase I treatment to ensure absence of genomic DNA
RNA lengths can reach over 12kb, indicating the intactness. High efficiency reverse transcription Representative of total RNA.
Applications:
Suitable for use in Northern Blot, RT-PCR, RACE, cDNA synthesis, cDNA library construction, cDNA probe for profiling study in gene expression, RNA protection, primer extension, RNA display, reverse Northern Blot and In vitro translation.
Storage and Stability:
For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.
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