Tissue, mRNA, Human Disease, liver cirrhosis, Spleen, BioGenomics(TM)

mRNA is isolated from total RNA obtained from human adult and fetal normal tissues, human tumor and diseased tissues, as well as from mouse, rat, monkey, and plant tissues. The RNA undergoes two rounds of oligo(dT) selection utilizing proprietary technology to minimize the presence of ribosomal RNA. Potential DNA contaminants are removed by a stringent DNase treatment. Contamination by RNase, DNA polysaccharides and proteoglycans has been effectively eliminated. The mRNA is purified by affinity chromatography using oligo dT columns. The purity and intactness of the mRNA is verified by agarose gel electrophoresis, Northern analysis, cDNA synthesis, and capillary electrophoresis using a human beta-actin cDNA probe.\n\nFeatures:\nmRNA isolated from a wide variety of tissues and cells \nDNase I treatment to ensure absence of genomic DNA \nRNA lengths can reach over 12kb, indicating the intactness \nHigh efficiency reverse transcription \nRepresentative of total RNA \nDocumentation of tissues' clinical histories is available (additional cost)\n\nApplications:\nNorthern Blotting, RT-PCR, and RACE \ncDNA synthesis and cDNA library construction \ncDNA probe for profiling study in gene expression \nRNA protection and primer extension \nRNA display and reverse Northern blotting \nIn vitro translation \n\nStorage Conditions:\nStore mRNA at -70C

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