mRNA is isolated from total RNA obtained from human adult and fetal normal tissues, human tumor and diseased tissues, as well as from mouse, rat, monkey, and plant tissues. The RNA undergoes two rounds of oligo(dT) selection utilizing proprietary technology to minimize the presence of ribosomal RNA. Potential DNA contaminants are removed by a stringent DNase treatment. Contamination by RNase, DNA polysaccharides and proteoglycans has been effectively eliminated. The mRNA is purified by affinity chromatography using oligo dT columns. The purity and intactness of the mRNA is verified by agarose gel electrophoresis, Northern analysis, cDNA synthesis, and capillary electrophoresis using a human beta-actin cDNA probe.
Features:
mRNA isolated from a wide variety of tissues and cells
DNase I treatment to ensure absence of genomic DNA
RNA lengths can reach over 12kb, indicating the intactness
High efficiency reverse transcription
Representative of total RNA
Documentation of tissues' clinical histories is available (additional cost)
Applications:
Northern Blotting, RT-PCR, and RACE
cDNA synthesis and cDNA library construction
cDNA probe for profiling study in gene expression
RNA protection and primer extension
RNA display and reverse Northern blotting
In vitro translation
Storage Conditions:
Store mRNA at -70C
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