Tissue Plasminogen Activator (tPA)

Tissue-type plasminogen activator (tPA) is one of two major physiologic activators of plasminogen in plasma. It is a serine protease of 68kD produced primarily in endothelial cells. It is also present in monocytes and megakaryocytes. Normal plasma tPA antigen concentrations have been reported from 20ng/ml-5ug/ml, depending on the assay used, but typically most of the tPA (> 90%) is in complex with its primary inhibitor, plasminogen activator inhibitor-1 (PAI-1). Structurally, tPA is a single-chain enzyme that consists of a catalytic domain followed by two kringle structures, an EGF domain and a finger domain. The activation of plasminogen by tPA is dependent on the presence of a fibrin cofactor. The binding of both tPA and plasminogen to fibrin is mediated in part through lysine binding sites within the kringle structures of both enzyme and substrate, but also through the finger domain of tPA. Activation of plasminogen by tPA occurs by cleavage after residue Arg560 to produce the two-chain active serine protease plasmin. The activity of tPA is regulated in part by a very short half life in circulation (t

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SPECIFICATIONS

Catalog Number

T5600-05

Size

500ug

Applications

ELISA

Hosts

Sheep

Reactivities

Hum

Form

Supplied in 10mM HEPES, pH 7.4, 0.15M sodium chloride, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1.Bachmann, F.: The Plasminogen-Plasmin Enzyme System; in Hemostasis and Thrombosis, 3rd Edition, eds. R.W. Colman, J. Hirsh, V.J. Marder, E.W. Salzman, pp. 1592-1622, J.B. Lippincott Co., Philadelphia PA, USA (1994). 2. Holvoet, P., Cleemput, H., Collen, D.: Assay of Human Tissue-Type Plasminogen Activator (t-PA) with an Enzyme-Linked Immunosorbent Assay (ELISA) Based on Three Murine Monoclonal Antibodies to t-PA. Thrombosis and Haemostasis 54: 684-687 (1985). 3. Giles, A.R., Nesheim, et al.: The fibrinolytic Potential of the Normal Primate following the Generation of Thrombin In Vivo. Thrombosis and Haemostasis 63: 476-481 (1990).

Additional Info

Recognizes humanTissue Plasminogen Activator (tPA). Monospecific when analyzed by immunoelectrophoresis. Does not crossreact with normal human plasma. Identity was verified using purified tPA.