Tissue Plasminogen Activator (tPA)

Hybridoma: \nHybridization of NSO cells with spleen cells from a BALB/c mouse immunized with purified melanoma-type t-PA\n\nApplications:\nSuitable for use in ELISA and for biochemical studies.\n\nRecommended Dilution: \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T5600-11G

Size

500ug

Hosts

Mouse

Form

Supplied as a lyophilized powder in PBS, pH 7.4, 20mg/ml mannitol, 0.02% sodium azide. Reconstitute to a final volume of 0.5ml.

P Type

Mab

Purity

Purified from murine ascites by affinity chromatography on immobilized recombinant Protein A. The purity of the product is tested by Laemmli SDS-PAGE.

Isotype

IgG1

Additional Info

Binds to t-PA. Reaction with an epitope expressed on Kringle 2. Competitive inhibition of plasminogen activation in the presence of fibrin (Ki = 0.57nM). Half maximal binding to coated t-PA at 19ug/ml (low affinity).