Home  >  Products  >  Titin (TTN, Cardiomyopathy Dilated 1G (Autosomal Dominant), CMD1G, CMH9, CMPD4, Connectin, DKFZp451N061, FLJ26020, FLJ26409, FLJ32040, FLJ34413, FLJ39564, FLJ43066, HMERF, LGMD2J, MU-RMS-40.14, Rhabdomyosarcoma Antigen MU RMS 40.14, TMD)
Titin (TTN, Cardiomyopathy Dilated 1G (Autosomal Dominant), CMD1G, CMH9, CMPD4, Connectin, DKFZp451N061, FLJ26020, FLJ26409, FLJ32040, FLJ34413, FLJ39564, FLJ43066, HMERF, LGMD2J, MU-RMS-40.14, Rhabdomyosarcoma Antigen MU RMS 40.14, TMD)

Titin (TTN, Cardiomyopathy Dilated 1G (Autosomal Dominant), CMD1G, CMH9, CMPD4, Connectin, DKFZp451N061, FLJ26020, FLJ26409, FLJ32040, FLJ34413, FLJ39564, FLJ43066, HMERF, LGMD2J, MU-RMS-40.14, Rhabdomyosarcoma Antigen MU RMS 40.14, TMD)

Cat no: T5650-02

Supplier: United States Biological
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Monoclonal Anti-Titin can be used for study of the elastic filaments within sarcomeric structures. It is also useful as a differentiation marker in the separation of rhabdomyosarcomas from other muscle tumors. By indirect immunofluorescence the antibody displays a typical striated staining pattern on frozen sections of chicken skeletal and cardiac muscle tissues. Stains the region of the A-I junction by indirect immunofluorescence. It shows a decoration line 0.05 mm from the end of the A band in electron micro-graphs.In immunoblotting, using total extracts of chicken breast muscle, the antibody reacts specifically with both bands of the titin double and shows no reaction with nebulin. Applications: Suitable for use in Electron microscopy, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (frozen): 1:2000 Immunofluorescence (IC): 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: T5650-02
Reactivities: Human, Rat, Chicken/Bird
Hosts: Mouse
Applications: Immunofluorescence, Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a liquid in ascites buffer, 0.1% Na azide
P type: Mab
Isotype: IgG2b
Purity: Ascites
References: 1. Furst DO et al. The organization of titin filaments in the half-sarcomere revealed by monoclonal antibodies in immunoelectron microscopy: a map of ten nonrepetitive epitopes starting at the Z line extends close to the M line. J Cell Biol 106:1563-72 (1988).
Additional info: Recognizes chicken titin. Localizes (connectin) in skeletal and heart muscle of a wide variety of species from cold-blooded vertebrates to human. Does not crossreact with nebulin, smooth muscle or different non-muscle tissues and cultured cells. Species Crossreactivity: human, mouse, rat, avian, fish and mammal.

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