TLR2 (Toll Like Receptor 2, CD282)

Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. TLR2 has been identified as a receptor \nthat is central to the innate immune response to lipoproteins of gram-negative bacteria, several whole gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. A functional interaction between TLR2 and TLR6 in the cellular response to various bacterial products has been discovered. The currently accepted paradigm regards TLR2 as an essential receptor for many eubacterial cell wall components, including lipoproteins and peptidoglycan. Bacterial species as diverse as mycobacteria, \nspirochetes, mycoplasma, Staphylococcus aureus, and Streptococcus pneumoniae have all been shown to mediate cellular activation via TLR2. \n\nApplications:\nSuitable for use in ELISA, Immunohistochemistry and Flow Cytometry. Not suitable for use in Western Blot or Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry: 1:50\nFlow Cytometry: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T8050-09N

Size

100ug

Applications

ELISA, FC, IHC

Hosts

Mouse

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

References

1. Meng, G et al; Murine TLR2 expression analysis and systemic antagonism by usage of specific monoclonal antibodies. Immunology Letters 2005, 98: 200

Additional Info

Recognizes mouse TLR2. Reacts with native TLR2 and does not react with denatured TLR2. Stains overexpressed, as well as endogenous cell surface- and intracellular mouse TLR2. Inhibits murine TLR2-mediated cell activation. Masks the lipopeptide binding site in the recombinant extracellular domain of TLR2 and does not hinder the receptor complex formation.

Alternative Names

TLR2, CD282