TLR2 (Toll Like Receptor 2, CD282, TIL4) (FITC)

Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. TLR2 has been identified as a receptor that \nis central to the innate immune response to lipoproteins of Gram-negative bacteria, several whole Gram-positive bacteria, as well as a receptor for peptidoglycan and lipoteichoic acid and other bacterial cell membrane products. A functional interaction between TLR2 and TLR6 in the cellular response to various bacterial products has been discovered. The currently accepted paradigm regards TLR2 as an essential receptor for many eubacterial cell wall components, including lipoproteins and peptidoglycan. Bacterial species as diverse as mycobacteria, spirochetes, mycoplasma, Staphylococcus aureus, and Streptococcus pneumoniae have all been shown to mediate cellular activation via TLR2 (CD282). \n\nApplications:\nSuitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunohistochemistry, and Immunoassay. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:50\nFlow Cytometry: 1:50\nWestern Blot: 1:50\nImmunohistochemistry: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nFor long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

T8050-09Z

Size

100ug

Applications

FC, IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Can

Form

Supplied as a liquid in PBS, 1% BSA. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Flo, T et al; Differential expression of Toll-like receptor 2 in human cells. J Leukoc Biol 2001, 69: 474 2. Schjetne, K et al; Link between innate and adaptive immunity: Toll-like receptor 2 internalizes antigen for presentation to CD4+ T cells and could be an efficient vaccine target. J Immunol 2003, 171: 32 3. Siedlar, M et al; Tolerance induced by the lipopeptide Pam3Cys is due to ablation of IL-1R-associated kinase 1. J Immunol 2004, 173: 2736 4. Tunheim, G et al; Human receptors of innate immunity (CD14, TLR2) are promising targets for novel recombinant immunoglobulin-based vaccine candidates. Vaccine 2007, 25: 4723 5. Burgener, I et al; Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol 2008, 124; 184

Additional Info

Recognizes human TLR2. Species crossreactivity: dog.

Alternative Names

CD282, Toll-like receptor 2