TLR2 (Toll Like Receptor 2, CD282, TIL4)

Toll-like receptors (TLR) are highly conserved throughout evolution and are implicated in the innate defense to many pathogens. Mammalian TLRs have been identified as type I transmembrane signaling receptors with pattern recognition capabilities. TLRs recognize pathogen-associated molecular patterns (PAMPs), expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. \n\nAmong TLRs, TLR2 is an unique receptor recognizing lipoproteins of Gram-negative bacteria, several whole Gram-positive bacteria, as well as peptidoglycan, lipoteichoic acid and other bacterial cell membrane products. A functional interaction between TLR2 and TLR6 in the cellular response to various bacterial products has been discovered. Bacterial species as diverse as mycobacteria, spirochetes, mycoplasma, Staphylococcus aureus, and Streptococcus pneumoniae have all been shown to mediate cellular activation via TLR2 (CD282).\nTLR2 is highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Furthermore, TLR2 is detected in lung and fetal liver. In other tissues TLR2 levels are low.\n\nApplications:\nSuitable for use in Immunofluorescence, Flow Cytometry, Immunoprecipitation, and inhibition of biological activity. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:50\nFlow Cytometry: 1:50\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8050-09S

Size

100ug

Applications

FC, IF, IP

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% BSA.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Nakao, Y et al; Surface-expressed TLR6 participates in the recognition of diacylated lipopeptide and peptidoglycan in human cells. J Immunol 2005, 174: 1566. 2. Uehori, J et al; Dendritic cell maturation induced by muramyl dipeptide (MDP) derivatives: Monoacylated MDP confers TLR2/TLR4 activation. J Immunol 2005, 174: 7096. 3. Funami, K et al; The cytoplasmic 'linker region' in Toll-like receptor 3 controls receptor localization and signaling. Int Immunol 2004, 16: 1143. 4. Matsumoto, M et al; Subcellular localization of Toll-like receptor 3 in human dendritic cells. J Immunol 2003, 171: 3154. 5. Uehori, J et al; Simultaneous blocking of human Toll-like receptors 2 and 4 suppresses myeloid dendritic cell activation induced by Mycobacterium bovis bacillus Calmette-Gu

Additional Info

Recognizes human TLR2 (CD282).