TLR3 (CD283) (APC)

Human TLR3 is a 116kD type I transmembrane glycoprotein that belongs to the mammalian Toll-Like Receptor family of pathogen pattern recognition molecules.1 TLR3 is found in phagosomes, where the acidic pH enables binding of internalized double-stranded RNA and mRNA from viruses, parasites, and necrotic virally-infected cells.2-6 Ligand binding by TLR3 induces receptor dimerization, leading to the release of inflammatory cytokines and dendritic cell maturation.3, 4, 6, 8 TLR3 is expressed in dendritic cells, macrophages, microglia, and astrocytes, and is upregulated by IFN-beta and LPS.4, 8-10 TLR3 expression is also induced by lung fibroblasts and epithelial cells by respiratory syncytial virus infection.7 The extracellular domain (ECD) of human TLR3 shares 80%, 79%, and 77% aa sequence identity with the ECD of rat, mouse, and bovine TLR3, respectively.\n\nApplications: \nSuitable for use in Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at 12 months at 4 degrees C. Freezing allophycocyanin conjugates will result in a substantial loss of activity. Allophycocyaninconjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

145633

Size

100Tests

Applications

FC

Hosts

Goat

Form

Supplied as a liquid in PBS, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Sen, G.C. & S.N. Sarkar (2005) Cytokine Growth Factor Rev. 16:1. 2. Nishiya, T. et al. (2002) J. Biol. Chem. 280:37107. 3. de Bouteiller, O. et al. (2005) J. Biol. Chem. 280:38133. 4. Alexopoulou, L. et al. (2001) Nature 413:732. 5. Aksoy, E. et al. (2005) J. Biol. Chem. 280:277. 6. Kariko, K. et al. (2004) J. Biol. Chem. 279:12542. 7. Rudd, B.D. et al. (2005) J. Virol. 79:3350. 8. Farina, C. et al. (2005) J. Neuroimmunol. 159:12. 9. Heinz, S. et al. (2003) J. Biol. Chem. 278:21502. 10. Wang, T. et al. (2004) Nat. Med. 10:1366.

Additional Info

Recognizes human TLR3.