TLR3 (Toll-like Receptor 3, CD283) (Biotin)

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1) and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described (3). TLR3 cDNA codes for a protein with approximate molecular weight of 120kD (4). TLR3 has a restricted expression pattern being expressed in dendritic cells (DC) (4-6). TLR3 mRNA expression was detected by in situ hybridization in DC and lymph nodes (6). The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting.\n\nApplications:\nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8050-21F

Size

100ug

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse, Can

Conjugates

Biotin

Form

Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with Biotin.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Muzio M, Bosisio D, Polentarutti N, D'amico G, Stoppacciaro A, Mancinelli R, van't Veer C, Penton-Rol G, Ruco LP, Allavena P, Mantovani A. J Immunol 164(11):5998-6004 (2000). 2. Muzio M, Polentarutti N, Bosisio D, Prahladan MK, Mantovani A. J Leukoc Biol 67(4):450-456 (2000). 3. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. Exp. Med. 187: 2097-2101 (1998). 4. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997). 5. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2): 157-161 (2001) 6. Rock FL., Hardiman G., Timans JC., Kastelein RA. and Bazan JF. Proc. Natl. Acad. Sci. U.S.A. 95 (2), 588-593 (1998).

Additional Info

Recognizes human TLR3. Species Crossreactivity: mouse and canine.