TLR3 (Toll-like Receptor 3, CD283)

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and an IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1) and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described (3). TLR3 has a restricted expression pattern being expressed in dendritic cells (DC) (4-6). TLR3 mRNA expression was detected by in situ hybridization in DC and lymph nodes (6). The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-3ug/ml\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T8050-20S

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Moll

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Medzhitov, R. and Janeway, CA. Cell. 91:295-298 (1997). 2. Rock, FL. et al. Proc. Natl. Acad. Sci. U.S.A. 95:588-593 (1998). 3. Muzio, M. et al. J. Exp. Med. 187:2097-2101 (1998). 4. Muzio, M. et al. J Immunol. 164:5998-6004 (2000). 5. Chuang, TH. and Ulevitch, RJ. Biochim. Biophys. Acta. 1518:157-161 (2001).

Additional Info

Recognizes human TLR3. Species Crossreactivity: chimpanzee, gorilla, macaque, rhesus monkey. Species sequence homology: feline, bovine, canine, equine, mouse, rat.