TLR3 (Toll Like Receptor 3) (FITC)

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1) and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described (3). TLR3 cDNA codes for a protein with approximate molecular weight of 120kD (4). TLR3 has a restricted expression pattern being expressed in dendritic cells (DC) (4-6). TLR3 mRNA expression was detected by in situ hybridization in DC and lymph nodes (6). The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting.\n\nApplications: \nSuitable for use in Western Blot and Flow Cytometry. Other applications not tested. \n\nRecommended Dilution: \nWestern Blot: 1-3ug/ml\nFlow Cytometry: 2-5ug/1x10e6 Ramos cells for intracellular staining.\nOptimal dilutions to be determined by researcher.\n\nPositive Control: Human intestine tissue lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8050-21A

Size

100ug

Applications

FC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in TBS, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A., J. Exp. Med . 187: 2097-2101 (1998). 2. Medzhitov R and Janeway CA., Cell 91: 295-298 (1997). 3. Chuang TH and Ulevitch RJ., Biochim. Biophys. Acta 1518 (1-2): 157-161 (2001). 4. Rock FL., Hardiman G., Timans JC., Kastelein RA. and Bazan JF., Proc. Natl. Acad. Sci . U.S.A. 95(2): 588-593 (1998). 5. Muzio M, Polentarutti N, Bosisio D, Prahladan MK, Mantovani A., J Leukoc Biol 67(4): 450-456 (2000). 6. Muzio M, Bosisio D, Polentarutti N, D'amico G, Stoppacciaro A, Mancinelli R, van't Veer C, Penton-Rol G, Ruco LP, Allavena P, Mantovani A., J Immunol 164(11): 5998-6004 (2000).

Additional Info

Recognizes human TLR3 (Toll Like Receptor 3). Species Crossreactivity: Human. Does not crossreact with mouse.