TLR4 (Toll Like Receptor 4, CD284) (FITC)

Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. TLR4 (CD284) has been identified next to MD-2 and CD14 as a receptor that is central to the innate immune response to lipopolysaccharides (LPS) of Gram-negative bacteria. \n\nApplications:\nSuitable for use in ELISA, Flow Cytometry, Functional Studies, Immunofluorescence, Immunoprecipitation and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: Use 10 ul of the neat solution to label 10e6 cells or 100 ul whole blood. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nFor long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

T8050-29G

Size

100ug

Applications

ELISA, FC, IF, IP, WB

Hosts

Mouse

Reactivities

Hum, Can, NHP

Form

Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Shimazu, R et al; MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J Exp Med 1999, 189: 1777 2. Tabeta, K et al; Toll-like receptors confer responsiveness to lipopolysaccharide from porphyromonas gingivalis in human gingival fibroblasts. Infect Immun 2000, 68: 3731 3. Akashi, S et al; Regulatory roles for CD14 and phosphatidylinositol in the signaling via toll-like receptor 4-MD-2. Biochem Biophys Res Commun 2000, 268: 172 4. Wang, JE et al; Involvement of CD14 and toll-like receptors in activation of human monocytes by Aspergillus fumigatus hyphae. Infect Immun 2001, 69: 2402 5. Triantafilou, M et al; Lateral diffusion of toll-like receptors reveals that they are transiently confined within lipid rafts on the plasma membrane. J Cell Sci 2004, 117: 4007 6. Burgener, I et al; Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol 2008, 124; 184

Additional Info

Recognizes human TLR4 (CD284) that is associated with MD-2. TLR4 (CD284) is a function-blocking antibody that is useful for studies on the role of TLR4 (CD284) as a receptor for LPS induced cytokine production by TLR4 bearing cells. Species crossreactivity: canine, cynomolgus monkey, rhesus monkey and marmoset monkey.

Alternative Names

CD284, Toll-like receptor 5