TLR4 (Toll Like Receptor 4, CD284)

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. Ten human homologs of TLRs (TLR1-10) have been described. Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling. The TLR4 cDNA codes for a protein consisting of 799 amino acids with approximate molecular weight of 88kD. \n\nApplications: \nSuitable for use in Flow Cytometry, Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Confocal Microscopy and Neutralization Assay. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry (Cell Surface): 2-3ug/1x10e6 (tested on PBMCs)\nImmunoprecipitation: see ref 8 for details\nFA(Neutralization): see ref 9 for details.\nImmunofuorescence/Immunocytochemistry: see ref 10 for details \nOptimal dilution to be determined by the researcher.\n\nRecommended Positive Control: PBMCs or ThP1\n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol 40-50%, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8050-29B

Size

100ug

Applications

FC, ICC, IF, IP

Hosts

Mouse

Reactivities

Hum, Mouse, Can

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G chromatography

Isotype

IgG2a,k

References

1. Muzio M, et al. J. Exp. Med. 187: 2097-2101 (1998). 2. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997). 3. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta. 1518: 157-161 (2001). 4. Takeuchi O, et al. Immunity . 11: 443 (1999). 5. Poltorak A, et al. Proc. Natl. Acad. Sci USA. 97:2163-2167 (2000). 6. Medzhitov R, et al. Nature. 388: 394-397 (1997). 7. Akashi S, et al. Biochem Biophys Res Commun. 268:172-177 (2000). 8. Shimazu, R., et al. J. Exp. Med. (1999). 189:1777-1782. 9. Paik YH, et al. Hepatology. 2003 37:1043-1055. 10. Tracy A, et al. J. Immunol. 175:376-384 (2005).

Additional Info

Recognizes human TLR4 (Toll Like Receptor 4). Has been shown to block the activation of monocytes with LPS. Species Crossreactivity: canine and mouse