TLR4 (Toll Like Receptor 4, Toll-like Receptor 4 Precursor, TLR 4, ARMD10, CD284, CD284 Antigen, hToll, TOLL) (Biotin)

Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defenses. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. TLR4 has been identified next to MD-2 and CD14 as a receptor that is central to the innate immune response to lipopolysaccharides (LPS) of Gram-negative bacteria. The HTA125 monoclonal antibody reacts preferentially, especially in Flow Cytometry, with human TLR4 that is associated with MD-2. HTA125 is a TLR4 function-blocking antibody that is useful for studies on the role of TLR4 as a receptor for LPS induced cytokine production by TLR4 bearing cells. The antibody was shown to precipitate TLR4 (100kD). \n\nApplications: \nSuitable for use in ELISA, Flow Cytometry, Functional Studies, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: Frozen sections\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T8050-31G1

Size

500ul

Applications

ELISA, FC, IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Can, NHP

Conjugates

Biotin

Form

Supplied as a liquid in PBS, 0.02% sodium azide, 0.1% BSA. Conjugated to Biotin.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Shimaz, R. et al., J. Exp. Med. 1999; 189: 1777-1782 2. Tabeta, K. et al., Infect. Immun. 2000; 68: 3731-3735. 3. Akashi, S. et al., Biochem. Biophys.. Res. Commun. 2000; 268: 172-177. 4. Wang, J.E. et al., Infect. Immun. 2001; 69: 2402-2406.

Additional Info

Recognizes human TLR4. Species Crossreactivity: canine, cynomolgus monkey, rhesus monkey and marmoset monkey.

Alternative Names

Homolog of Drosophila Toll, Toll (Drosophila) Homolog, Toll Endotoxin Hyporesponsiveness