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TLR4 (Toll Like Receptor 4, Toll-like Receptor 4 Precursor, TLR 4, CD284, CD284 Antigen, Homolog of Drosophila Toll, hToll, Toll (Drosophila) Homolog, TOLL, Toll Endotoxin Hyporesponsiveness)

TLR4 (Toll Like Receptor 4, Toll-like Receptor 4 Precursor, TLR 4, CD284, CD284 Antigen, Homolog of Drosophila Toll, hToll, Toll (Drosophila) Homolog, TOLL, Toll Endotoxin Hyporesponsiveness)

Cat no: T8050-31F

Supplier: United States Biological
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TLR4 has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. Applications: Suitable for use in Flow Cytometry, Functional Assays, and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:10-1:50; 10ul labels 10e6 cells in 100ul Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: T8050-31F
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Immunoprecipitation
Size: 500ug
Form: Supplied as a liquid in PBS. No preservative added.
P type: Mab
Isotype: IgG2a
Purity: Purified by Protein G affinity chromatography.
Additional info: Recognizes the human Toll like receptor 4 (TLR4). Species Crossreactivity: Rhesus Monkey, Guinea Pig, Porcine, Canine

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