Home  >  Products  >  TLR4 (Toll Like Receptor 4, Toll-like Receptor 4 Precursor, TLR 4, CD284, CD284 Antigen, Homolog of Drosophila Toll, hToll, Toll (Drosophila) Homolog, TOLL, Toll Endotoxin Hyporesponsiveness)
TLR4 (Toll Like Receptor 4, Toll-like Receptor 4 Precursor, TLR 4, CD284, CD284 Antigen, Homolog of Drosophila Toll, hToll, Toll (Drosophila) Homolog, TOLL, Toll Endotoxin Hyporesponsiveness)

TLR4 (Toll Like Receptor 4, Toll-like Receptor 4 Precursor, TLR 4, CD284, CD284 Antigen, Homolog of Drosophila Toll, hToll, Toll (Drosophila) Homolog, TOLL, Toll Endotoxin Hyporesponsiveness)

Cat no: T8050-33F

Supplier: United States Biological
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The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Among this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 835 amino acids with approximate molecular weight of 90kD (6). Applications: Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Positive Control: RAW or thioglycolate-elicited mouse macrophage cells Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: T8050-33F
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry, Immunoprecipitation
Size: 100ug
Form: Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
P type: Mab
Isotype: IgG2a,k
Purity: Purified by Protein G affinity chromatography.
References: 1. Poltorak A, Riccardi-Castagnoli P, Citterio S, and Butler B. Proc. Natl. Acad. Sci USA 97: 2163-2167 (2000). 2. Akashi S, Shimazu R, Ogata H, Nagai Y, Takeda K, Kimoto M, Miyake K. J Immunol 164(7):3471-5 (2000). 3. Takeuchi O., Hoshino K, Kawai T, Sanjo H, Takada H, Ogawa K, Takeda K and Akira S. Immunity 11: 443 (1999). 4. Medzhitov R, Preston-Hurlburt P and Janeway CA Jr. Nature 388 (6640), 394-397 (1997). 5. Chuang TH and Ulevitch RJ. Biochim. Biophys. Acta 1518 (1-2): 157-161 (2001). 6. Muzio M, Natoli G, Saccani S, Levrero M, and Mantovani A. J. Exp. Med. 187: 2097-2101 (1998). 7. Medzhitov R and Janeway CA. Cell 91: 295-298 (1997).
Additional info: In FA, the antibody blocks activation of monocytes with LPS (Akashi et al. 2000). This antibody preferentially recognizes TLR4-MD-2 complex than of TLR4 alone. The optimal condition has to be determined for individual experiments. Species Crossreactivity: Mouse.

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