TLR9, Fc Chimera, Recombinant, Mouse, aa26-818 (Toll-like Receptor 9, CD289)

TLR9 (TollпїЅlike receptor 9), designated CD289, is a member of the TLR family of innate immune receptors that is mainly expressed by colonic epithelium, CD123+ plasmacytoid predendritic cells (pDC), and splenic transitional B cells (1-9). TLR9 responds to unmethylated DNA CpG motifs that occur mainly in bacteria and viruses (1, 2). Mouse TLR9 cDNA encodes a 1032 amino acid (aa) type I transmembrane glycoprotein with a 793aa extracellular domain (ECD) that contains 26 leucineпїЅrich repeats (LRRs, aa 26пїЅ818), and a 193aa cytoplasmic domain with a TIR sequence that dimerizes with signaling adaptors such as MyD88 (1). The mouse TLR9 ECD shares 87% aa sequence identity with rat and 71-74% with human, feline, canine, equine, porcine, bovine and ovine TLR9. Predicted splice forms vary at the N-terminus by initiating either upstream or downstream of the standard site. The full-length 150kD form, which is ligand-binding but nonsignaling, is found in the endoplasmic reticulum. It undergoes accessory protein-mediated translocation either to the cell membrane or to lysosomes (1-3). TLR9 is cleaved to remove LRR1-14, producing an 80kD signaling fragment within acidic endolysosomes where it encounters microbial CpG DNA rather than selfпїЅDNA (2, 10, 11). However, immune complexes of self-DNA with lupus erythematosus antiпїЅDNA antibodies can induce TLR9 activation and IFN-alpha production in pDC (4). A soluble form also found in endosomes includes all 26 LRRs and negatively regulates active TLR9 (12). Activation of TLR9 contributes to splenocyte proliferation, pDC maturation, macrophage inflammatory cytokine production, Th1 inflammatory responses, NK cell activation and recruitment, B cell surface MHC class II up-regulation and immunoglobulin production, and generation and maintenance of memory B cells (1, 5-9).\n\nSource:\nRecombinant protein corresponding to aa22-734 from human Semaphorin 4D Fc Chimera, CHO-derived.\n\nMolecular Weight:\n~140-170kD\n\nEndotoxin:\n<0.10EU/1ug (LAL method)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute in sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

145847

Size

50ug

Form

Supplied as a lyophilized powder in PBS. BSA free. Reconstitute in 100ug/ml PBS.

Purity

~95% (SDS-PAGE)

References

1. Hemmi, H. et al. (2000) Nature 408:740. 2. Barton, G.M. et al. (2006) Nat. Immunol. 7:49. 3. Latz, E. et al. (2004) Nat. Immunol. 5:190. 4. Barrat, F.J. et al. (2005) J. Exp. Med. 202:1131. 5. Krieg, A.M. et al. (1995) Nature 374:546.\n6. Aranburu, A. et al. (2010) J. Immunol. 185:7293. 7. Guerrier, T. et al. (2012) J. Autoimmun. 39:173. 8. Guillerey, C. et al. (2012) Blood 120:90. 9. Cunningham