TNF alpha BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human TNF alpha in cell culture supernates, serum and plasma (heparin, EDTA, citrate).\n\nTumor necrosis factor-alpha (TNF-alpha, or TNF) is secreted by macrophages in response to inflammation, infection and cancer.1 Human Tumor Necrosis Factor (TNF) and Lymphotoxin (TNF-beta) are cytotoxic proteins which have similar biological activities and share 30% amino acid homology.2 TNF-alpha is produced by monocytes, which can stimulate endothelial cells to produce the multilineage growth factor granulocyte-macrophage colony-stimulating factor and extend the role of this immunoregulatory protein to the regulation of hematopoiesis in vitro.3 TNF is a soluble protein that causes damage to tumor cells but has no effect on normal cells. Human TNF has been purified to apparent homogeneity as a 17.3-kilodalton protein from HL-60 leukemia cells and has showed cytotoxic and cytostatic activities against various human tumor cell lines. The human TNF cDNA is 1585 base pairs in length and encodes a protein of 233 amino acids. The mature protein begins at residue 77, leaving a long leader sequence of 76 amino acids.4 TNF-alpha has been mapped to human chromosome 6.2\n\nAssay Range:\n15.6pg/ml-1000pg/ml (Body fluids, tissue lysates or cell culture supernates), 7.8pg/ml-500pg/ml (Human serum)\n(Body fluids, tissue lysates or cell culture supernates)\n7.8pg/ml-500pg/ml\n(Human serum)\n\n\nSensitivity:\n< 1 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP01375\n\nEntrez Gene ID:\n7124\n\nKit Components:\n96-well plate pre-coated with anti-TNF alpha, Human\nLyophilized recombinant TNF alpha, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

144065

Size

96Tests

References

1. Brenner, D. A.; O'Hara, M.; Angel, P.; Chojkier, M.; Karin, M. Prolonged activation of JUN and collagenase genes by tumour necrosis factor-alpha. Nature 337: 661-663, 1989. \n2. Nedwin, G. E.; Naylor, S. L.; Sakaguchi, A. Y.; Smith, D.; Jarrett-Nedwin, J.; Pennica, D.; Goeddel, D. V.; Gray, P. W. Human lymphotoxin and tumor necrosis factor genes: structure, homology and chromosomal localization. Nucleic Acids Res. 13: 6361-6373, 1985. \n3. Broudy, V. C.; Kaushansky, K.; Segal, G. M.; Harlan, J. M.; Adamson, J. W. Tumor necrosis factor type alpha stimulates human endothelial cells to produce granulocyte/macrophage colony-stimulating factor. Proc. Nat. Acad. Sci. 83: 7467-7471, 1986. \n4. Wang, A. M.; Creasey, A. A.; Ladner, M. B.; Lin, L. S.; Strickler, J.; Van Arsdell, J. N.; Yamamoto, R.; Mark, D. F. Molecular cloning of the complementary DNA for human tumor necrosis factor. Science 228: 149-154, 1985.\n