TNFAIP3, Recombinant, Human, FLAG-Tag (Tumor Necrosis Factor Alpha-induced Protein 3, TNF Alpha-induced Protein 3, OTU Domain-containing Protein 7C, Putative DNA-binding Protein A20, Zinc Finger Protein A20, OTUD7C)

Tumor necrosis factor, alpha-induced protein 3 (TNFAIP3) is a de-ubiquitinating enzyme that contains an N- terminal catalytic domain belonging to the ovarian-tumor superfamily of cysteine proteases (1). TNFAIP3 suppresses apoptosis.It is expressed in various cell types in response to a variety of stimuli that activate the transcription factor NF- kB, including Il-1, CD40 ligand, and PMA (3). TNFAIP3 has been strongly suggested as a key protein involved in tamoxifen resistance, and thus represents both a new breast cancer marker and a promising target for developing new strategies to prevent the emergence of acquired mechanisms of drug resistance in breast cancer (2).\n\n\nSource:\nRecombinant corresponding to aa2-791 from human TNFAIP3, fused to FLAG-tag at N-terminal, expressedin Sf9 insect cells via a baculovirus expression system.\n\nMolecular Weight:\n~90.5kD\n\nApplications: \nSuitable for for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137384

Size

50ug

Form

Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 0.05% Tween-20, 80ug/ml FLAG peptide, 20% glycerol, 3mM DTT.

Purity

Purified (~10%)

References

1. Evans P.C., et al. Biochem. J. 378:727-734, 2004 2. Vendrell JA, et al. Oncogene 26(32):4656-67, 2007 3. Daniel S., et al. Blood 104(8):2376-84, 2004. 4 Shi CS, Kehrl JH. (2010). Autophagy 6(7):986-7. 5. Tavares, R.M., et al. (2010). Immunity 33(2):181-9. 6. Hymowitz, S.G., Wertz, I.E. (2010). Nat. Rev. Cancer 10(5):332-41.