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Tnfsf10, Recombinant, Mouse (Tumor Necrosis Factor Ligand Superfamily Member 10, TNF-related Apoptosis-inducing Ligand, Protein TRAIL, CD253, Trail, A330042I21Rik, AI448571)

Cat no: 149712

Tnfsf10, Recombinant, Mouse (Tumor Necrosis Factor Ligand Superfamily Member 10, TNF-related Apoptosis-inducing Ligand, Protein TRAIL, CD253, Trail, A330042I21Rik, AI448571)

TRAIL is a cytotoxic protein, which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL induced apoptosis is achieved through binding to two death-signaling receptors, DR4 and DR5. These receptors belong to the TNFR superfamily of transmembrane proteins and contain a cytoplasmic пїЅdeath domainпїЅ, which activates the cell's apoptotic machinery. Recombinant murine soluble TRAIL is a 174aa polypeptide, 20kD, consisting of the TNF homologous portion of the extracellular domain of the full length TRAIL protein.\n\nSource:\nRecombinant protein corresponding to mouse TRAIL, expressed in E.coli.\n\nBiological Activity:\nDetermined by the dose-dependent stimulation of MIP-2 production by mouse spleen cells using a concentration range of 10-100ng/ml.\n\nEndotoxin:\n<0.1ng/ug (1EU/ug)\n\nAA Sequence:\nMRGGRPQKVA AHITGITRRS NSALIPISKD GKTLGQKIES WESSRKGHSF LNHVLFRNGE LVIEQEGLYY IYSQTYFRFQ EAEDASKMVS KDKVRTKQLV QYIYKYTSYP DPIVLMKSAR NSCWSRDAEY GLYSIYQGGL FELKKNDRIF VSVTNEHLMD LDQEASFFGA FLIN\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

149712

Size

10ug

Form

Supplied as a lyophilized powder.

Purity

~98% (SDS-PAGE and HPLC)

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