TNFSF4/OX40L BioAssay(TM) ELISA Kit, Mouse

Sandwich ELISA quantitative detection kit for Mouse TNFSF4/OX40L in cell culture supernates, serum and plasma (heparin, EDTA).\n\nOX40L is the ligand for CD134 and is expressed on such cells as DC2s enabling amplification of Th2 cell differentiation. OX40L has also been designated CD252. By analysis of an interspecific backcross, Baum et al. (1994) mapped the mouse Tnfsf4 gene to chromosome 1. Using fluorescence in situ hybridization, they localized the human TNFSF4 gene to 1q25, a region sharing homology of synteny with the portion of mouse chromosome 1 containing the Tnfsf4 gene.OX40L strongly inhibited the generation of IL-10-producing Tr1 cells induced by two physiologic stimuli, the inducible costimulatory ligand and immature dendritic cells. In addition, OX40L strongly inhibited IL-10 production and suppressive function of differentiated IL-10-producing Tr1 cells. Tnfsf4 underlies Ath1 in mice and that polymorphisms in its human homolog TNFSF4 increase the risk of myocardial infarction in humans.\n\nAssay Range:\n62.5pg/ml-4000pg/ml\n\nSensitivity:\n < 10 pg/ml \n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP43488\n\nEntrez Gene ID:\n22164\n\nKit Components:\n96-well plate pre-coated with anti-TNFSF4/OX40L, Mouse\nLyophilized recombinant TNFSF4/OX40L, Mouse standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

144082

Size

96Tests

References

1. Baum, P. R., Gayle, R. B., III, Ramsdell, F., Srinivasan, S., Sorensen, R. A., Watson, M. L., Seldin, M. F., Baker, E., Sutherland, G. R., Clifford, K. N., Alderson, M. R., Goodwin, R. G., Fanslow, W. C. Molecular characterization of murine and human OX40/OX40 ligand systems: identification of a human OX40 ligand as the HTLV-1-regulated protein gp34. \n2. Maizels RM, Yazdanbakhsh M (2003). \"Immune regulation by helminth parasites: cellular and molecular mechanisms\". Nat. Rev. Immunol. 3 (9): 733