TNFsR? BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human TNF s R? in cell culture supernates, serum and plasma (heparin, EDTA, citrate and urine.\n\nTNFsR?(also known as Solube tumor necrosis factor receptor 1) is a cytokine receptor that binds tumor necrosis factors (TNF). In contrast, the p60 (TNFRSF1A) and p80 (TNFRSF1B) TNFA receptors self-assemble through a distinct functional domain in the TNFR extracellular domain, termed the pre-ligand assembly domain (PLAD), in the absence of ligand. Deletion of the PLAD results in monomeric presentation of p60 or p80. Flow cytometric analysis showed that efficient TNFA binding depends on receptor self-assembly. They also found that other members of the TNF receptor superfamily, including the extracellular domains of TRAIL (TNFRSF10A), CD40, and FAS (TNFRSF6), all self-associate but do not interact with heterologous receptors. By Southern blot analysis of human/Chinese hamster somatic cell hybrid DNA, the TNFR1 gene was mapped to 12pter-cen. And by nonradioactive in situ hybridization that the type 1 receptor (the p55 TNF receptor) is encoded by a gene located on chromosome 12p13.2.\n\nAssay Range:\n7.8pg/ml-500pg/ml\n\nSensitivity:\n< 1 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP19438\n\nEntrez Gene ID:\n7132\n\nKit Components:\n96-well plate pre-coated with anti-TNFsR?, Human\nLyophilized recombinant TNFsR?, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

144083

Size

96Tests

References

1 Chan, F. K.-M., Chun, H. J., Zheng, L., Siegel, R. M., Bui, K. L., Lenardo, M. J. A domain in TNF receptors that mediates ligand-independent receptor assembly and signaling. Science 288: 2351-2354, 2000.\n2. Milatovich, A., Song, K., Heller, R. A., Francke, U. The genes for two tumor necrosis factor receptors, TNFR1 and TNFR2, map to human chromosomes 12 (12pter-cen) and 1 (1pter-p32). Cytogenet. Cell Genet. 58: 1980-only, 1991. \n3. Derre, J., Kemper, O., Cherif, D., Nophar, Y., Berger, R., Wallach, D. The gene for the type 1 tumor necrosis factor receptor (TNF-R1) is localized on band 12p13. Hum. Genet. 87: 231-233, 1991. \n