TOMM22 (Translocase of Outer Membrane 22kD Subunit Homolog, Translocase of Outer Mitochondrial Membrane 22 Homolog, TOM 22, TOM22, TOM-22, 1C9 2, 1C9-2, hTom22, Mitochondrial Import Receptor Subunit TOM 22 Homolog, Mitochondrial Import Receptor Subunit TO

Tom22 has a negatively charged N-terminal region exposed to the cytosol, a putative transmembrane region, and a C-terminal intermembrane space region with little negative charge. The following functions can be assigned to the three domains of Tom22: the cytosolic N-terminal domain plays a dual role, specifically important for presequence binding; the intermembrane space domain provides a trans binding site for presequences, and the single membrane anchor of Tom22 is crucial for the integrity of the GIP (general import gene) complex. In the absence of this membrane anchor, the GIP complex dissociates into small core complexes containing a dimer of Tom40 and the three small Toms. A preprotein is stably arrested and accumulated in the GIP complex by Tom40 and Tom22.4 Such a 100kD core complex probably contains a single channel that retains the basic channel properties but is already open in the absence of preproteins. In contrast, in the presence of Tom22, the wild-type GIP complex contains tightly regulated channels (probably three channels). Tom22 apparently represents a component of the machinery that controls the gate. The cytosolic domains of Tom22 and Tom20 are believed to form the major part of a cis site, which mediates the import of all preproteins known to use the general import machinery of mitochondria. The preprotein is then routed through the Tom complex translocation channel and transferred to a trans site on the intermembrane space (IMS) side of the outer membrane. The inter-membrane space exposed segment of Tom40 and the C-terminal tail of Tom22 may contribute to the trans-site. Matrix-targeted proteins are further transferred to the matrix through Product Information import machinery in the inner membrane. The TOM complex of mammalian mitochondria resembles the fungal Tom complex, but is distinct from the plant TOM system. Thus, while unique components of the mammalian mitochondrial import system have been identified (e.g. TOM34 and metaxin), Tom22, and Tom37 have not been identified in plant mitochondria.\n\nApplications: \nSuitable for use in Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: Raji whole cell lysate.\n\nCellular Localization: Mitochondrial\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T8064-21

Size

50ul

Applications

ICC, WB

Hosts

Mouse

Reactivities

Hum, NHP

Form

15mM Sodium Azide; 1% BSA, 0.01M PBS. pH 7.4

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

Saeki K et al. Identification of mammalian TOM22 as a subunit of the preprotein translocase of the mitochondrial outer membrane. J Biol Chem 275:31996-2002 (2000). <PUBMED:10900208>Suzuki H et al. Characterization of rat TOM40, a central component of the preprotein translocase of the mitochondrial outer membrane. J Biol Chem 275:37930-6 (2000). <PUBMED:10980201>Yano M et al. Identification and functional analysis of human Tom22 for protein import into mitochondria. Mol Cell Biol 20:7205-13 (2000). <PUBMED:10982837>

Additional Info

Species Crossreactivity: Crossreacts with Human and Monkey. Not yet tested in other species.