DNA topoisomerase 2-alpha is a 170kD member of the type II topoisomerase family. Human TOP2A is 1531aa in length, and splicing variants produce three additional isoforms which have substitutions following Lys321, Gln355, or Ala401. TOP2A contains a histidine kinase-like ATPases domain (aa80-193), a TopoIIA Trans ScTopoIIA domain (aa265-417), a TOPRIM TopoIIA: topoisomerase-primase domain (aa455-575), a DNA Topoisomerase IV domain (aa697-1169), and a NES (aa1018-1028). There are multiple potential Ser/Thr phosphorylation sites. Human TOP2A shares 89aa sequence identity with mouse TOP2A.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution:
Western Blot: 0.5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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